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The putative TrpA1 mRNA from M. sexta is expressed in the lateral and medial styloconic sensilla. RT-PCR for TrpA1 was performed on tissue samples containing both classes of sensilla. The expected 205-bp fragment was amplified from tissue samples (arrow; compare with indicated size standards, Roch ME ladder VIII). Reverse transcriptase was omitted in samples labeled –RT and included in those labeled +RT.
