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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Clin Cancer Res. 2013 May 29;19(13):3416–3428. doi: 10.1158/1078-0432.CCR-13-0073

Figure 2.

Figure 2

Knockdown of RRM2 induces apoptosis via the mitochondria-mediated intrinsic pathway. (A) Tu212 (left panel) and A549 (right panel) cells were transfected with siC or siR2. After 72 h, cell lysates were analyzed by Western blotting with the indicated antibodies. A single set of blots is pictured from three independent experiments. (B) Tu212 (upper panel) and A549 (lower panel) cells were transfected with siC or different siRNAs against RRM2, siR2, siR2-1, siR2-2. After 72 h, cell lysates were analyzed by Western blotting with the indicated antibodies. A single set of blots is pictured from three independent experiments. (C) Mitochondrial fraction (MF) and cytosolic fraction (CF) of Tu212 and A549 cells were isolated 72h after transfection with 5 nM siR2, 5 nM siC, or no treatment (NT). Western blotting was performed to detect Cytochrome C and Cox-4.