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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Clin Cancer Res. 2013 May 29;19(13):3416–3428. doi: 10.1158/1078-0432.CCR-13-0073

Figure 4.

Figure 4

Apoptosis induction by knockdown of RRM2 is p53-, p73-, and Akt-independent. (A) Western blotting for p53 and p73 expression in Tu212 and A549 cells 72h after transfection with siC or siR2. (B) Western blotting for the indicated proteins in A549 and p53-knockdown A549 (A549/sh p53) cell lines after transfection with 5nM siC or siR2. A representative blot of three independent experiments is presented. (C) Apoptosis analysis (error bars are mean ±SD from 3 independent experiments). (D) Western blot analysis 72h after transfection with 5nM of siC or siR2 in H1299 and two clones of dominant negative p73-expressing H1299 (H1299/dN p73 Cl-7, Cl-10) cell lines. (E) Western blotting for p-Akt and Akt in Tu212 and A549 cells 72h after transfection with siC or different concentrations of siR2. (F) Apoptosis analysis. (G) Western blot of whole cell lysates from A549 and Akt-overexpressing A549 (A549/Akt) cell lines 72 h post-siRNA transfection.