Figure 5.

FRS2α is essential for FGF-induced cell migration. (A) Migration assay using Boyden chamber was performed with wild-type fibroblasts, FRS2α-deficient fibroblasts, or FRS2α-deficient fibroblasts infected with expression vectors for FRS2α or vector alone. The fibroblasts were stimulated with 10% serum ░⃞, FGF1 (100 ng/ml) and heparin (5 μg/ml) ■, PDGF (50 ng/ml) ▨, or EGF (100 ng/ml) ▤ or medium alone □. (B) Fibroblasts were stimulated with FGF1 (100 ng/ml) and heparin (5 mg/ml) ■ or medium alone □. (C) Migration analysis of unstimulated □ or FGF-stimulated ■ FRS2α-deficient fibroblasts that were transfected with expression vectors for FRS2α (WT), for the 2F, 4F, or 6F mutants, or with vector alone.