Fig. 5.

Characterization of apomixis-associated DETs identified by transcriptomic analysis in Paspalum genotypes. Top left: a representative example of DET (apo417/N114) identified in P. notatum flowers at late pre-meiotic/meiotic stage by differential display experiments. Differential display amplicons originating from three apomictic and three sexual F₁ plants (in duplicate) were transferred to nylon membranes and hybridized with an N114 probe labelled with digoxigenin (Pessino et al., 2001). Top right: a representative example of a Class A DET identified in P. simplex flowers by cDNA-AFLP analysis at pre-anthesis, anthesis and post-anthesis stages (Polegri et al., 2010). Bottom: histogram representing the relative occurrence of DETs belonging to particular ontology classes in P. notatum, as identified from DD experiments and in P. simplex, as identified from cDNA-AFLP experiments. Ontology classes (from left to right): transposon/retrotransposon, signal transduction, protein metabolism, miRNA precursors, general metabolism, transcription, cell cycle, transport, cytoskeleton, other, unknown.