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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Hepatology. 2012 Dec;56(6):2071–2081. doi: 10.1002/hep.25904

Figure 1. Identification of six intrahepatic antigen presenting cell (APC) subsets by multi-parameter flow cytometry.

Figure 1

A. Interstitial mononuclear cells isolated from an HCV-infected liver were simultaneously stained with three, overlapping, 10-color antibody panels differing from each other by an individual marker. Antibodies were directed against CD3, CD56, CD19, CD14, CD16, HLA-DR, CD11c, CD123, CD303, CD1c, CD141, CD34, and CD33. APC subsets were identified among a parental population of HLA-DR+ cells following exclusion of dead cells and those cells expressing lineage markers CD3, CD56 and CD19. B. Six dominant subsets of intrahepatic APCs identified among HLA-DR+ cells are depicted, including: CD303+ plasmacytoid DCs (CD303+, CD11c−, CD123++, CD1c−, CD303+, HLA-DR+), CD1c+ myeloid DCs (CD1c+, CD11c++, CD14+/−, CD123+, HLA-DR++), CD16+ CD14+ cells (CD16+, CD14+, CD11c++, CD123+, HLA-DR+), CD16− CD14+cells (CD14++, CD16−, CD11c++, CD123+, HLA-DR+), CD34+ cells (CD11c+, CD141+/−, CD14+, CD123+, CD34++, CD33+/−, HLA-DR+) and CD141+ myeloid DCs (CD141++, CD11c+, CD14−, CD123+, CD34−, CD33+, HLA-DR+). Phenotypic characterization of each population is shown in the corresponding red (APC subset) and grey (total lineage negative cells) overlays.

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