Figure 15.

“Smart immobilization” methods for reproducible, high-sensitivity, multiplexed, high-throughput microfluidic immunoassays, or enzyme assays. (a) Photoactivated protein immobilization, (b) electrochemically activated immobilization, (c) thermally activated immobilization, (d) photoactivated protein elution, (e) spatially addressable multiplexed protein immobilization, (f) reversible protein immobilization for repetitive assay, and (g) multifunctional immobilization surface for simplified immobilization procedure.