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In situ polymerized nylon membrane for an enzyme assay. (a) In situ polymerization of nylon membrane using organic/aqueous two-phase flow in an X-shaped microfluidic chip, and (b) TLM (thermal-lens microscopy) was used to detect product and substrate. Reprinted with permission from H. Hisamoto et al., Anal. Chem. 75, 350 (2003). Copyright 2003 American Chemical Society.
