Fig. 6.

Co-localization of HP1α and its mutants, W174A and W41A, in the nuclei of GHFT1 cells. (a) to (c) Confocal images of Cerulean3-HP1α(WT), Cerulean3-HP1αW174A and Cerulean3-HP1αW41A expressed alone (column 1), or when co-expressed with mApple-BZip (columns 2 to 4). For two color imaging with no signal crosstalk, the Cerulean3 signal was obtained by excitation with the 448 nm laser line, while using the $480/40$ emission filter. The mApple signal was then acquired at the same focal plane by excitation with the 561 nm laser line, while using the $609/54$ emission filter; the scale bar is 5 μm.