Table 2.

FCS analysis of the diffusion for mCerulean3 and mCerulean3 fusion proteins.

Species	Power at the objective (μW)a	$D$ (${\mu \mathrm{m}}^2{\mathrm{s}}^{-1}$)b	SD	Number of trialsc
mCer3 in solution	0.37	79	6	6
mCer3 in solution	0.91	83	6	8
mCer3 in solution	1.68	82	7	7
mCer3 in solution	2.25	87	5	4
mCer3 in solution	3.11	89	4	8
mCer3 in live cells	1.68	23	5	28
mCer3-BZip in the nucleus	1.68	7.1	1.7	8
mCer3-BZip + mApple-HP1α	1.68	7	1.5	10
mCer3-HP1α in the nucleus	1.68	4.8	1.1	22
mCer3-HP1α + mApple-BZip	1.68	4.9	1.4	16

^aPower at the objective was determined as described in Sec. 2.4.

^bCoefficient of diffusion $D$ was determined using Eq. (3) by a Levenberg-Marquardt minimization of the autocorrelation curves after using a standard dye to determine beam waist and shape factor (as described in Sec. 2.4). For mCer3-BZip and mCer3-HP1α, Eq. (3) does not capture the complexity of the diffusion profiles, as discussed in the text.

^cThe number of trials is the number of readings obtained for that experiment. For live cell experiments, these readings were obtained from a minimum of 3 cells. For trials involving two colors, the red channel was monitored to ensure that the concentration of co-expressed mApple proteins was equivalent to or greater than the concentration of mCerulean3 proteins being detected.