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rSir3p assembles 12-mer nucleosomal arrays into supramolecular nucleoprotein complexes. (A) Intact 208–12 NA, (B) 208–12 DNA, and (C) trypsinized 208–12 NA were incubated at the indicated rSir3p, and samples were electrophoresed on a 1.0% agarose gel (1× TBE) as described under Materials and Methods. Arrowheads indicate the size of selected markers in base pairs.
