Figure 2.

UV/Vis absorption spectrum of a plate-like crystal (50 µm × 20 µm × 10 µm) of partially reduced horse heart myoglobin. To minimize morphological artifacts, the crystal was rotated along ω to yield best absorption spectra. Acquisition time was 100 × 2.6 ms with an entrance slit width of 100 µm, using a 300 lines mm⁻¹ grating and a UV-optimized EMCCD detector. The wavelength range in the UV is accessible down to at least 250 nm.