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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: J Biomol NMR. 2013 Mar 29;55(4):391–399. doi: 10.1007/s10858-013-9724-z

Figure 4.

Figure 4

Resolution improvement for EmrE using the reverse labeling approach in conjunction with 2D FDR-NCA experiments. (A) [U-13C,15N] EmrE standard NCA 2D dataset using SPECIFIC-CP (same as in Figure 2). (B) RevIL EmrE standard NCA 2D dataset using SPECIFIC-CP and acquired in a similar manner as that in panel A. (C) FDR-NCA spectrum of RevIL EmrE. The spectrum in panel C shows only peaks for residues immediately following Ile and Leu in the primary sequence. Panels A, B, and C are plotted at 7.1σ, 5.8σ, and 7.0σ, respectively.