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. 2001 Jul 10;98(15):8590–8595. doi: 10.1073/pnas.151240998

Figure 4.

Figure 4

A conserved methionine residue is essential for the activity of the vesiculoviral M proteins. Wild-type or mutant GST-M proteins were injected into the nucleus 1 h before injection of export substrates. Export of 32P-labeled U1Sm− snRNA was monitored 1 and 3 h after RNA injection. (A) RNA export was analyzed in the absence and presence of GST-tagged M proteins of VSV, VSV(M51L), SVCV, SVCV(M31L), CV, and CV(M54L). (B) RNA export was analyzed as in A in the presence of GST-tagged M proteins of VSV(D52N), SVCV(D32N), and CV(E55A).