Figure 2. Scanning electron microscopy of the somatic embryo development in Coffea canephora.

A) Proembryogenic mass (Pm) from a leaf explant at 21 days after induction (dai). B) Globular stage; the white arrowhead indicates the protoderm establisment. C) Heart stage; the white arrows indicate the beginning of the cotyledonary primordia in the embryo. D) Torpedo stage; the white arrow indicates the enlargement of the embryo. E) Early cotyledonary stage, where the establishment of the future cotyledons can be observed. F) Late cotyledonary stage. At this stage the cotyledons are fully developed. Bars = 100 µm.