A) Expression of LEC1, BBM1 and WOX4 was performed from total RNA samples for RT-PCR analysis that were isolated from leaf explants under embryogenic induction at days 0, 7, 14 and 21, and the different embryogenic stages were isolated and comparatively classified in proembryogenic mass (Pm), globular (G), heart (H), torpedo (T), cotyledonary (C) and zygotic embryo (Z) in cotyledonary stage. UBQ11 was used as a reference gene. B) Densitometric analysis of the gene expression showed in A. Relative expression of LEC1, BBM1 and WOX4 were normalized to the constitutive gene UBQ11. Different letters in columns represent the statistical significance of mean differences at a given time according to the Tukey test (P≤0.05). Each RT-PCR was conducted twice with three independent biological replicates.