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. 2013 Aug 20;8(8):e72581. doi: 10.1371/journal.pone.0072581

Figure 3. Fluorescence in situ hybridizations of Spirostomum sp. 72 and Euplotes octocarinatus FL(12)-VI.

Figure 3

Spirostomum sp. 72 was fixed with 4% osmium tetroxide (a-b-c) and Euplotes octocarinatus FL(12)-VI was fixed with 4% formaldehyde in PBS (d-e-f). (a) signal of Cy3-labeled probe MegPol436, specific for 'Candidatus Megaira polyxenophila' (macronuclei enhanced); (b) signal of Fluorescein-labeled probe EUB338 (macronuclei enhanced); (c) merged image, (a) + (b); (d) phase contrast; (e) signal of Fluorescein-labeled probe Rick_527, targeting members of the Rickettsiaceae family; (f) merged image, (d) + (e). Bar: 10 µm.