Skip to main content
. 2013 Aug 20;8(8):e71601. doi: 10.1371/journal.pone.0071601

Figure 1. Schematic map and restriction enzyme digests of pHS501, pRC501 and pHS121 plasmid DNA isolated from rBCG.

Figure 1

(A) Schematic map of E. coli/mycobacterial shuttle vector. (B) Lanes 1 & 8 contain the molecular weight marker GeneRulerTM 1 kb Ladder (Fermentas, S.A.). Lanes 2 & 3 contain pHS501 plasmid DNA. Lanes 4 & 5 contain pRC501 plasmid DNA. Lanes 6 & 7 contain pHS121 plasmid DNA. Lanes 2, 4 & 6 contain plasmid DNA isolated prior to transformation into BCG (positive controls). Lanes 3, 5 & 7 contain plasmid DNA isolated from rBCG. Plasmid DNA in lanes 2 to 7 was digested with restriction enzymes XbaI and HpaI. Results were the same for rBCG and rBCGΔpanCD. Enzymatic restriction analysis of only 2 representative samples are shown for each rBCG, however plasmid DNA was isolated from a minimum of 20 of each rBCG.