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. 2013 Aug 20;8(8):e71493. doi: 10.1371/journal.pone.0071493

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© 2013 Bonney et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A, B) Pathway analysis comparing wildtype and Per2^−/− mice after 30 minutes of ischemia without reperfusion. Differentially regulated genes and pathways were analyzed using Partek and Ingenuity software, respectively. (C) Fatty acid subpopulation analysis in wildtype and Per2^−/− hearts at baseline using nuclear magnet resonance (NMR) technique. Representative NMR spectra for total lipids or monounsaturated fatty acids (MUFAs) at baseline are displayed. To calculate an absolute monounsaturated fatty acid (MUFA) concentration, the concentration of polyunsaturated fatty acids, triacylglycerides and glycerides are subtracted from the total for this peak. (D) WT or Per2^−/− mice were exposed to 30 minutes of ischemia without reperfusion. Shock frozen hearts were analyzed for total lipid and MUFA content at baseline (B) and Ischemia (I) using NMR, n = 3 mice in all groups.