Figure 5. Lamin A mutations V415I and Q432X trigger nuclear deformations in heat shock experiments.

C2C12 murine myoblast cells transiently expressing GFP WT, V415I or Q432X lamin A were exposed to a 15 minute heat shock at 42°C. After fixation the nuclear morphology was analysed by immunofluorescence (A) and statistically evaluated (B). Cells expressing GFP lamin A WT were used for reference. 600 nuclei each were analysed. Cells expressing GFP lamin A Q432X showed significant nuclear deformations already before the heat shock. P-Values of less than 0,001 are defined as highly significant (**).