Table 4. Primers.
| Primer | Primer sequences | Genotype |
| 5′UTR Ex F1 | CCCTGTGAGGAACT(AT)CTGTCTTCACGC | All |
| 5′UTR In F3 | TCTAGCCATGGCGTTAGT(AG)(CT)GAG | All |
| Core Ex R2 | GGTGCACGGTCTACGAGACCT | All |
| Core In R4 | CACTCGCAAGCACCCTATCAGGCAGT | All |
| NS5B Ex F | TGGGGATCCCGTATGATACCCGCTGCTTTGA | 1–5 & ‘7’ |
| NS5B Ex R | CGGAATTCCTGGTCATAGCCTCCGTGAA | 1–5 & ‘7’ |
| NS5B ExF G6 | CCHATGGGGTTYTCCTAYGACAC | 6 |
| NS5B ExR G6 | GGNGCYGAGTAYCTGGTCATGGC | 6 |
| NS5B In F | GACACCCGCTGCTTTGACTC | All |
| NS5B In R | GAGTCTTCACGGAGGCTATGACNAGGTA | All |
| 860 Ex F | GCGCTTATCGCATACGGCTAG | rs12979860 |
| 860 Ex R | CCCAGCAGGCGCCTCTCCTA | rs12979860 |
| 860 In F | CCTGGACGTGGATGGGTACTG | rs12979860 |
| 860 In R | GCAGGCGCCTCTCCTATGTCAG | rs12979860 |
| 917 Ex F | CATACAACATGGAGAGTTAAAGTAAGTC | rs8099917 |
| 917 Ex R | GCTGGCCCCAGGAGCTTGCACTAG | rs8099917 |
| 917 in R | CCTGTGCTGGGCCACCACAATTCA | rs8099917 |
Primers used in RT-PCR, and PCR reactions of the 5′UTR and NS5B region of HCV and the nested amplifications of the IL28B SNPs. Genotype 7 is in quotations as it is yet to be confirmed as a new genotype. TaqMan SNP typing for rs8099917 was conducted using custom Assays-on-Demand products from Applied Biosystems (C__11710096_10) and verified with the listed primers, using standard PCR and sequencing techniques.