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. 2013 Aug 20;8(8):e71233. doi: 10.1371/journal.pone.0071233

Table 1. Primer sequences for amplification of cf76.

Primer Sequence Product
cf76 Forward ORF 5′ ACGACAAGCATATGCTCGAG- ATGAAATTTTTATTAATGTTTGTGGTGCCTTTG 3′ Full length cf76 (2172 bp)
cf76 Reverse ORF 5′ TCCGGAACATCGTATGGGTA- AACTAGTGTTAATGATAACAATAATGTAGC 3′ Full length cf76 (2172 bp)
cf76 Forward Fragment 1 5′ ACGACAAGCATATGCTCGAG-ATGAAATTTTTATTAATGTTTGTGGTGCCTTTG 3′ N-terminal region (720 bp)
cf76 Reverse Fragment 1 5′ TCCGGAACATCGTATGGGTA- TTCCACTTGAGGTCCAGTGACTATAC 3′ N-terminal region (720 bp)
cf76 Forward Fragment 2 5′ ACGACAAGCATATGCTCGAG-GATCGTGGCGGAAGTATAGTCACTG 3′ Central region (828 bp)
cf76 Reverse Fragment 2 5′ TCCGGAACATCGTATGGGTA- AGCTATTGAATGTTCTTCTTGTAATGAATT 3′ Central region (828 bp)
cf76 Forward Fragment 3 5′ ACGACAAGCATATGCTCGAG-GAAGAACATTCAATAGCTAATTCATTA 3′ C-terminal region (675 bp)
cf76 Reverse Fragment 3 5′ TCCGGAACATCGTATGGGTA- AACTAGTGTTAATGATAACAATAATGTAGC 3′ C-terminal region (675 bp)