cf76 Forward ORF |
5′ ACGACAAGCATATGCTCGAG- ATGAAATTTTTATTAATGTTTGTGGTGCCTTTG 3′
|
Full length cf76 (2172 bp) |
cf76 Reverse ORF |
5′ TCCGGAACATCGTATGGGTA- AACTAGTGTTAATGATAACAATAATGTAGC 3′
|
Full length cf76 (2172 bp) |
cf76 Forward Fragment 1 |
5′ ACGACAAGCATATGCTCGAG-ATGAAATTTTTATTAATGTTTGTGGTGCCTTTG 3′
|
N-terminal region (720 bp) |
cf76 Reverse Fragment 1 |
5′ TCCGGAACATCGTATGGGTA- TTCCACTTGAGGTCCAGTGACTATAC 3′
|
N-terminal region (720 bp) |
cf76 Forward Fragment 2 |
5′ ACGACAAGCATATGCTCGAG-GATCGTGGCGGAAGTATAGTCACTG 3′
|
Central region (828 bp) |
cf76 Reverse Fragment 2 |
5′ TCCGGAACATCGTATGGGTA- AGCTATTGAATGTTCTTCTTGTAATGAATT 3′
|
Central region (828 bp) |
cf76 Forward Fragment 3 |
5′ ACGACAAGCATATGCTCGAG-GAAGAACATTCAATAGCTAATTCATTA 3′
|
C-terminal region (675 bp) |
cf76 Reverse Fragment 3 |
5′ TCCGGAACATCGTATGGGTA- AACTAGTGTTAATGATAACAATAATGTAGC 3′
|
C-terminal region (675 bp) |