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. Author manuscript; available in PMC: 2013 Aug 20.
Published in final edited form as: Exp Neurol. 2011 Oct 28;233(1):283–291. doi: 10.1016/j.expneurol.2011.10.017

Figure 4.

Figure 4

Identification of proliferating cells in the ischemic penumbra seven days following focal cerebral ischemia. Dual-IF with cell-specific markers and the proliferation marker Ki67 was performed on frozen sections of ischemic penumbra or contralateral hemisphere to identify: (A) endothelial cells (CD31), (B) astrocytes (GFAP), or (C) microglia/macrophages (Mac-1). White lines demarcate the ischemic core/penumbra boundary. Scale bar = 25μm (inserts = 7.5μm). Note that no cell proliferation was detected in the contralateral hemisphere. In contrast, the ischemic penumbra contained proliferating endothelial cells, astrocytes and microglia/macrophages.