Figure 6.

α₂δ₄subunit immunoreactivity was not observed in horizontal cells or their processes in the mouse retina. A: Single scan showing horizontal cells identified with calbindin (magenta) immunostaining and the α₂δ₄ subunit immunostaining (green). B–D: High magnification (boxed area from A) of a single scan showing the α₂δ₄ subunit immunoreactivity (C), calbindin (B), and an overlay of the two, demonstrating a lack of colocalization (D). E: Single scan showing horizontal cells tips labeled with a VGAT (magenta) and α₂δ₄subunit antibody (green). F–H: High magnification (boxed area from E) of a single scan showing VGAT immunostaining (F), α₂δ₄ (G), and overlay (H).Scale bar = 20 µm in A,E; 2 µm in B (applies to B–D) and F (applies to F–H).