Figure 8.

α₂δ₄ subunit immunoreactivity was observed in photoreceptor terminals of the mouse retina. A: α₂δ₄ subunit and protein kinase C (PKC) immunoreactivity in a rd/rd adult mouse retina that lacks photoreceptors (Carter-Dawson et al., 1978). B–D: High-magnification view of a single scan (boxed area from A). α₂δ₄ subunit immunoreactivity was significantly reduced in the rd/rd adult mouse OPL and some of the remaining α₂δ₄ subunit immunoreactive puncta (green) colocalized with rod bipolar cell terminals (arrows) immunostained with PKC antibodies (magenta). E: α₂δ₄ subunit and PKC immunoreactivity in cone-DTA mice. These mice lack cone photoreceptors (Soucy et al., 1998). F–H: High-magnification view of single scan (boxed area from E) showing α₂δ₄ subunit immunoreactive puncta distal to rod bipolar cell dendrites (arrows) suggesting the presence of the α₂δ₄ subunit at rod photoreceptor terminals. I: Labeling of cone pedicles with peanut agglutinin (PNA) conjugated to FITC (green) and α₂δ₄ subunit antibody (magenta) in the wild-type mouse. J,K: High-magnification view of single scan showing that α₂δ₄ subunit immunoreactivity is not located at the base of cone pedicles. Scale bar = 10 µm in A,E,I; 2 µm in B (applies to B–D), F (applies to F–H), and J (applies to J,K).