Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2014 Aug 1.

Published in final edited form as: Mol Cancer Res. 2013 May 10;11(8):923–936. doi: 10.1158/1541-7786.MCR-12-0686

Lack of effects of bone marrow miR-155 deficiency on macrophage infiltration in primary tumor. A, Representative fluorescence images of tumor tissues from WT and miR-155−/− chimeric mice at the time of primary tumor resection (14 days after implantation of LLC cells). DAPI was used to stain the nuclei of cells. Magnification, 10 ×. B, F4/80 cells (shown in green) were calculated as the ratio of green fluorescence protein-surface area to DAPI-surface area. Each of the six fields was averaged per mouse. C, Immunohistochemistry for vWF expression in primary tumor tissues from WT and miR-155−/− chimeric mice at day 14 after LLC inoculation. Magnification, 20 ×. D, Quantification of vWF microvessel density (MVD). vWF positive endothelial cell or cell cluster clearly separate from adjacent structures was considered a single microvessel. Each of the eight fields was averaged per mouse. Data are presented as the mean ± SEM of 8 mice.

Lack of effects of bone marrow miR-155 deficiency on macrophage infiltration in primary tumor. A, Representative fluorescence images of tumor tissues from WT and miR-155−/− chimeric mice at the time of primary tumor resection (14 days after implantation of LLC cells). DAPI was used to stain the nuclei of cells. Magnification, 10 ×. B, F4/80 cells (shown in green) were calculated as the ratio of green fluorescence protein-surface area to DAPI-surface area. Each of the six fields was averaged per mouse. C, Immunohistochemistry for vWF expression in primary tumor tissues from WT and miR-155−/− chimeric mice at day 14 after LLC inoculation. Magnification, 20 ×. D, Quantification of vWF microvessel density (MVD). vWF positive endothelial cell or cell cluster clearly separate from adjacent structures was considered a single microvessel. Each of the eight fields was averaged per mouse. Data are presented as the mean ± SEM of 8 mice.