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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Neurobiol Dis. 2013 May 20;58:57–67. doi: 10.1016/j.nbd.2013.05.007

Figure 5.

Figure 5

Ndufaf2 knockdown increases mitochondrial superoxide. a) Mitochondrial superoxide was labeled with MitoSOX, and fluorescence was quantified using flow cytometry (n=7). For rescue experiments, cells were pretreated with 100 μM Vitamin E for 18 h (n=3). Shown are median fluorescence intensities (mean+SEM, 1way ANOVA/Tukey's multiple comparison test.*, p<0.001, #, p<0.01). b) Control and Ndufaf2 shRNA SK-N-MC cells were grown on fibronectin-coated glass bottom dishes, stained in parallel with Mitotracker (green), MitoSOX (red) and CellMask (blue), and imaged live on a confocal microscope. Shown are representative images of three independent experiments. c) SDS-PAGE and Western-blot analysis of MnSOD in glycolytic (left panels) and oxidative (right panels) condition. Actin was used as a loading control. Quantifications were performed using densitometry (n=6, mean+SEM, unpaired student's T-Test.*,p<0.005, #, p<0.05)