Dopamine transporter (DAT) Immunohistochemistry: Effect of SN79 pretreatment on methamphetamine (METH)-induced decrease in striatal DAT levels. Male, Swiss Webster mice (N = 4/group) were pretreated with saline (Sal) or SN79 (SN, 3 mg/kg, i.p.). After 15 min, the mice were then treated with Sal or METH (5 mg/kg, i.p.). This treatment schedule was repeated four times at 2 h intervals. One week later, the brains were removed and stained for DAT immunoreactivity. Average optical density readings (mean ± SEM) are shown. ***P<0.001 vs. saline, ###P<0001 vs. METH.