Figure 4.

Two trajectories for floral anthocyanin gains in the Chilean Mimulus. (A) M. luteus has yellow flowers with red anthocyanic spots in the throat, typical of other closely related species in the genus. M. cupreus has gained anthocyanin throughout the corolla, giving it a red-orange color. (B) F2's from a cross between M. luteus and M. cupreus show that petal lobe anthocyanins segregate independently from variation in anthocyanic throat markings (Cooley and Willis, 2009). (C) M. variegatus is a second taxon that independently gained floral anthocyanins (Cooley et al., 2011). (D) Segregating variation in F2's between M. luteus and M. variegatus suggests that variation in anthocyanins may occur via repatterning of the spot on the lower-middle petal. Extensive variation in size and shape of this marking is evident, including examples where expansion of spots cover the entire distal half of each petal. All photos provided by Arielle Cooley.