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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Methods Mol Biol. 2013;1035:75–101. doi: 10.1007/978-1-62703-508-8_8

Figure 6. CD146 expression in primary stromal cells and its use in sorting stromal cells.

Figure 6

A and B: Immune Histochemistry (IHC) for CD146 expression of normal human bone marrow ( Panel A is an isotype antibody control. CD146 positive cells are present in a perivascular distribution, a location consistent with other models of where the HSPC niche might reside.

C: Flow-cytometry analysis of MSC cultures after 1 passage ( 10–14 days of culture). A variable proportion of cells are CD146 positive and they inhabit of continuum of antigen expression. Two MSCs set up from separate donors are shown.

D: Typical flow-sorting results from CD146-based sorting of MSCs. Approximately 35% of cells are deemed CD146 positive ( pre-sort, top histogram), and sorted to negative ( middle histogram) and CD146 negative ( bottom histogram) using a FACS-ARIA sorter ( Beckton Dickinson and Company) with a 100 μM nozzle.