| T75 Flask (or 100 mm dish): | 15–20 × 106 cells in 12 ml |
| T25Flask: | 5–10 × 106 cells in 5 ml |
| 6 well plate: | 3 × 106 cells in 2–3 ml/well |
| 24 well plate: | 1 × 106 in 1 ml/well |
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