Representative immunoblotting patterns reacted with the purified fusion-tagged MMP-like protein (A) compared with the fusion-tagged protein alone (B) expressed in Escherichia coli. Antigens were probed with 1:100 diluted sera from pooled negative controls (N), pooled human gnathostomiasis patients (P), healthy control (1, 2), parasitologically confirmed gnathostomiasis (3–7), clinically suspected visceral gnathostomiasis (8) and cutaneous gnathostomiasis (9), cysticercosis (10), angiostrongyliasis (11), paragonimiasis (12), fascioliasis (13), trichinosis (14), ascariasis (15), opisthorchiasis viverrini (16), strongyloidiasis (17), taeniasis (18), capillariasis (19), and hookworm infection (20). The arrow indicates the specific immunoreactive band at ∼102 kDa and arrow head indicates the fusion-tagged protein alone at ∼78 kDa.