Skip to main content
. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Biomaterials. 2013 Jun 17;34(28):6760–6772. doi: 10.1016/j.biomaterials.2013.05.066

Figure 2. ECM characterization of decellularized pancreas.

Figure 2

(A) IHC staining showed the presence of major ECM composition–Col I, Col IV, Fibronectin, laminin and phalloidin actin staining (green) in native pancreas. (B) Comparison with decellularized pancreas, IHC staining demonstrated the corresponding ECM markers found in native pancreas also preserved in decellularized markers but DAPI and actin cytoskeletal element were absent. This indicates complete removal of cellular materials but preservation of important ECM proteins. (C) Alcian blue staining qualitatively determined the retention of sGAG protein after decellularization. (D) Blyscan assays quantitatively determined the sGAG retention in decellularized pancreas was on average 52% of that in native pancreas (P<0.05).