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. Author manuscript; available in PMC: 2014 Aug 16.
Published in final edited form as: ACS Chem Biol. 2013 Jun 6;8(8):1794–1802. doi: 10.1021/cb400165b

Figure 1. Effect of heme on the expression of the heme utilization proteins.

Figure 1

RNA isolated from the indicated strains following 8 hours growth in media supplemented with heme was used as described in the Methods. mRNAs levels for PhuR (white bars), PhuS (black bars), HemO (dark grey bars), BphO (light grey bars) represent the standard deviation from at least three independent experiments in triplicate. (A) PAO1; (B) PAO1 ΔphuS; (C) PAO1 ΔhemO; and (D) PAO1 ΔphuS/ΔhemO. The indicated p-values were normalized to mRNA levels of the respective genes in the absence of heme where *p< 0.05, ** p< 0.005 or ***p<0.001.