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. Author manuscript; available in PMC: 2014 Aug 16.
Published in final edited form as: ACS Chem Biol. 2013 Jun 6;8(8):1794–1802. doi: 10.1021/cb400165b

Figure 5. LC-MS/MS BVIX isomer fragmentation patterns for PAO1 ΔhemO strain supplemented with 5 μM 13C-heme.

Figure 5

(A) HPLC analysis of BV isomers following extraction from the extracellular media. BVIX isomer peaks as marked. *Indicates a non-BVIX contaminant; (B) MS/MS fragmentation of 13C-BVIXα (red line) and 12C-BVIXα (black); (C) MS/MS fragmentation of 13C-BVIXδ (red line) and 12C-BVIXδ (black); MS/MS fragmentation of 13C-BVIXβ (red line) and 12C-BVIXβ (black). LC-MS/MS was performed as described in the Methods with multiple reaction monitoring.