Figure 3. Chromatin remodeling-defective yChd1 proteins are unable to generate arrays of evenly-spaced nucleosomes during chromatin assembly.
(A) Chromatin assembly reactions were performed with wild-type or mutant yChd1 proteins (30 nM) in the presence of AMP-PNP or ATP. The reaction products were subjected to partial MNase digestion analysis. (B) Chromatin assembly reactions were performed with the indicated concentrations of yChd1 proteins and then subjected to partial MNase digestion analysis.