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. Author manuscript; available in PMC: 2014 Jun 4.
Published in final edited form as: Anal Chem. 2013 May 22;85(11):5569–5576. doi: 10.1021/ac4008418

Figure 4.

Figure 4

BoNT/A ALISSA quantification of BoNT/A in mouse serum measured with 5A20.4 antibody on CNBr-activated sepharose beads. (A) BoNT/A activity in serum from mice (n=4) that were i.v. injected with 100, 20 or 4 pg/mouse dose of BoNT/A. A blood was withdrawn 1 h after intoxication. (B) Time course analysis of BoNT/A serum activity in mice (n=3) that were i.v. injected with 100 pg BoNT/A complex. (C) BoNT/A serum concentration measured over time in mice (n=3) that were orally administered 4 µg or (D) 1 µg of BoNT/A complex. Pre-injection serum (t = 0h) was used for background subtraction.