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Ni and CAP synergistically inhibit eNOS dimerization. Aorta from FA/CAP-exposed ApoE−/− mice were isolated and lyzed in RIPA buffer. The dimerization of eNOS and the phosphorylation of eNOS and Akt were analyzed with nondenaturing and denaturing gel electrophoresis. (A) The representative images of Western blot analysis. (B–D) The quantification of eNOS dimerization (B), eNOS phosphorylation (C), and Akt phosphorylation (D). n = 5–6/group. *p < 0.05 versus control; one-way ANOVA.
