Fig. 3.

Sublethal exposure causes abnormalities in bones of the F0 zebrafish head. Zebrafish larvae were exposed to TCDD at 50 pg/ml at 3 and 7 wpf and collected at 1 year of age for alizarin red staining as described in the Materials and Methods section. Photographs show lateral views of the live fish and the same view after digestion and staining, revealing the bones. Arrows point to the shortened jaw structures and to the shortened operculum in the live fish and to the altered underlying bone in the stained specimen.