Fig. 4.

TCDD causes altered sex ratio in F0 adults. Zebrafish larvae were exposed to TCDD at 50 pg/ml at 3 and 7 wpf as described in the Materials and Methods section. The grey box indicates percentage of females, whereas the open box represents percentage of males. The error bars indicate SEM. (A) Sex ratios were determined by visual appearance at 12 wpf. (B) Fish were dissected at 1 year of age, and sex was scored based on gonadal sex. Control, n = 52; 4 hpf, n = 58; 3 and 7 wpf, n = 50 individuals.