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. Author manuscript; available in PMC: 2014 Jun 20.
Published in final edited form as: Cell. 2013 Jun 20;153(7):1448–1460. doi: 10.1016/j.cell.2013.05.027

Figure 3. SIRT1 up-regulates circadian gene RNA levels in the SCN.

Figure 3

(A) Quantitative RT-PCR analysis of CLOCK-BMAL1 controlled genes. 6 month-old mice were sacrificed for brain samples at 4 hr intervals across the 12:12 light/dark cycle (n=3-4 mice/genotype/time point). Suprachiasmatic nucleus (SCN)-enriched anterior hypothalamus samples were later isolated for RNA preparation from frozen brain samples with needle punch collection. Target gene expression levels are shown relative to the ribosomal protein reference gene, rpl19. (B) Typical in situ hybridization images and relative signal intensities for Bmal1 and Per2 mRNA in the SCN. Sections were prepared from 3 month-old mice that were sacrificed at ZT2 and ZT14 (n≥3 mice/genotype). DAPI stained images were shown to indicate SCN in the BSKO sections. Signal intensities of protein levels were quantitated and are shown relative to wild type (≥ 6 sections). Values represent the mean ± standard deviation. *P<0.05; **P<0.01; t test.