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. Author manuscript; available in PMC: 2014 Jun 20.
Published in final edited form as: Cell. 2013 Jun 20;153(7):1448–1460. doi: 10.1016/j.cell.2013.05.027

Figure 5. SIRT1 regulates endogenous circadian gene RNA and protein levels in neuroblastoma N2a cells.

Figure 5

(A) Quantitative RT-PCR analysis of clock controlled genes. Mouse N2a cells (WT) and Sirt1 shRNA knock down cells were synchronized with 50% horse serum and time points were taken at 4 hr intervals. Results are shown relative to WT after normalization to ribosomal reference gene, rpl19. Values represent the mean ± standard deviation. *P<0.05; **P<0.01; t test. (B) Immunoblots of SIRT1 and components of the circadian machinery. Time points were taken at 6 h intervals after serum shock.