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. Author manuscript; available in PMC: 2014 Jun 20.
Published in final edited form as: Cell. 2013 Jun 20;153(7):1448–1460. doi: 10.1016/j.cell.2013.05.027

Figure 6. SIRT1 and PGC-1α mutually regulate circadian gene expression in N2a cells.

Figure 6

(A) Quantitative RT-PCR analysis of clock controlled genes. Sirt1 or Pgc-1α shRNA knock down cells and transient Sirt1 or Pgc-1α overexpression cells were synchronized with 50% horse serum 24 or 36 hr before harvesting for total RNA. Results are shown relative to a vector control. (B) Reporter assays using Bmal1- or Per2- luciferase under the indicated knock down or overexpression conditions. N2a cells were harvested for luciferase assays 24 hr after horse serum shock. Results are shown relative to a vector control. Values represent the mean ± standard deviation. *P<0.05; **P<0.01; t test. See also Figure S3 and S4.