Fig. 3.

Decreased collagen deposition in ECM after long-term culture. (a) Control and patient fibroblasts were maintained for 21 days after confluence. After medium removal, the cell layers were labeled with a polyclonal anti-type I collagen, LF-67, and nuclei were counterstained with DAPI. The fibrillar network is absent in patient 903. (b) Quantitation of collagen turnover in matrix, with the day 0 time point arbitrarily set to 100, showing similar rates of turnover between control (white) and patient cells (gray). (c) Comparison of α1(I) chain (left) and α2(I) chain (right) deposition and turnover of control and patient matrix, showing much less matrix deposited from patient 903 cells.