Figure 4.

Treatment with AEPU prevents nuclear translocation of NF-κB in primary murine neonatal cardiomyocytes. Cardiomyocytes treated with 50 µM Angiotensin II (Ang II) or phenylephrine (PE) were either cotreated with 20 µM AEPU or left untreated for 45 minutes. The nuclear translocation of p65 was visualized in fixed cells after staining with DAPI (blue) and anti-p65 mAb, followed by anti-mouse Texas red-conjugated secondary Ab (red). A Zeiss confocal microscope was used. Cells were serum starved for 24 hours and grown on coverslips. Adapted with permission from Xu et al.³