Figure 2.

Adenovirus vectors induce significant decreases in expression of proliferation-related Cyclin D1 (A) and proliferating cell nuclear antigen (PCNA; B) and an increase in expression of apoptosis-related BAX (C) in human uterine leiomyoma cells (HuLM). HuLM cells were transduced with Ad-LacZ, Ad-TK and Ad-SSTR-RGD-TK at 10 and 50 plaque-forming units (pfu)/cell and ganciclovir (GCV) at 10 µg/ml for 48 h. Lysates were analyzed by western blotting. The intensity of each protein signal was quantified and normalized with corresponding beta-actin. Data are mean ± SE, n = 3. *A significant difference from Ad-lacZ (P < 0.05, Student's t-test). The results are indicative of two independent experiments.