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. 2013 Jul 2;28(9):2398–2406. doi: 10.1093/humrep/det275

Figure 3.

Figure 3

The adenovirus vectors Ad-TK and Ad-SSTR-RGD-TK do not induce any significant changes compared with Ad-LacZ in either proliferation—proliferating cell nuclear antigen (PCNA) (A) and Cyclin D1 (B) or apoptosis— BAX (C) and antiapoptosis BCL-2 (D) related gene expression in human uterine smooth muscle cells (UtSM). UtSM cells were transduced with Ad-LacZ, Ad-TK and Ad-SSTR-RGD-TK at 10 and 50 plaque-forming units (pfu)/cell and GCV at 10 µg/ml for 48 h. Lysates were analyzed by western blotting with anti-PCNA, anti-Cyclin D1, anti-BAX and anti-BCL-2 antibodies. The intensity of each protein signal was quantified and normalized with corresponding beta-actin. Data are mean ± SE, n = 3. The results are indicative of three independent experiments.