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. 2001 Jul 10;98(15):8644–8649. doi: 10.1073/pnas.151253498

Figure 2.

Figure 2

Human BRCA2 promotes HR. (a) Schematic map of the chromosomal homologous-targeting substrates. Donation of wt xgprt sequence from the episomal pΔ3 to the chromosomally integrated pΔ2-puro through either gene conversion or crossover events restores xgprt function and confers resistance to XHATM. (b) Reconstitution of wt BRCA2 function in BRCA2-deficient Capan-1 cells enhances HR frequencies. Capan-1 cells with stable pΔ2-puro integration (Capan/pΔ2-puro) were transfected with donor plasmid pΔ3 and pcDNA3 based HA-BRCA2 expression plasmid (19) or control plasmid. The plating efficiency of cells was used to calculate the HR frequencies, i.e., HR frequency equals the number of XHATM-resistant colonies per total number of viable cells seeded. Logarithmic means of HR frequencies +/− SEM are based on four independent experiments.