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Growth suppression of self-renewing single GBM cells by SVV-001 in vitro. Single GBM cells were plated in clonal density in quadruplicates in 96-well plates and incubated in serum-free medium containing growth factors (EGF and bFGF) that favor the growth of CSCs. Formation of neurospheres was examined under a phase-contrast microscope, and cell viability was examined with a CCK-8 assay. *P < .05, **P < .01 compared with untreated control.
