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Subcellular localization of lipin1 in mast cells. WT BMMCs transduced with GFP-CD63 retrovirus were left unstimulated or stimulated with Ag at the indicated times. Lipin1 was stained using a rabbit anti-lipin1 antibody and detected with a secondary Texas Red-conjugated anti-rabbit antibody, followed by visualization under confocal microscopy. Scale bar represents 3μm. original magnification, 630 ×.
